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KMID : 1025520090510030193
Journal of Animal Science and Technology
2009 Volume.51 No. 3 p.193 ~ p.200
Commercial Application of Porcine MC1R Gene Polymorphisms to Korean Pork Industry
Ha You-Kyoung

Kim Kwan-Suk
Choi Yang-Il
Kim Sang-Wook
Jeon Soon-Hong
Choi Jae-Won
Choi Jung-Seok
Lee Seug-Soo
Abstract
The pork from black-coated pigs is famous among-consumers for better eating quality. The loci affecting black coat color was identified in pig chromosome 6 in which several genetic effects on pork quality have been reported. The melanocortin 1 receptor(MC1R) gene is a major gene which plays a key role in regulation of eumelanin(black/brown) and phaeomelanin (red/yellow). In this study, the MC1R gene polymorphism was analyzed for pig breed determination and genetic association with pork quality traits. MC1R Ala243Thr variation was analyzed to determine a specific genotype for four commercial pig breeds(Landrace, Yorkshire, Berkshire and, Duroc) and a Korean native pigs(KNP). Then we developed original KNP-specific DNA markers to determine the pork from black-coated pigs using MC1R DNA sequences. The total length of the MC1R coding sequence ranged 1451bp in KNP. KNP had the 0201 allele pertaining to ED1 but some of the KNP had the EP allele, probably reflecting the geneticintrogression of EPallele into KNP. Furthermore, a relationship between Leu102Pro single nucleotide polymorphism(SNP) genotype and pork quality phenotype were analyzed in F2 reciprocal-crossbred population between KNP and Yorkshire. Association analysis indicated that the allele of the MC1R gene has no effect on pork quality. These results suggest that black coat-color is not directly associated with preferred pork quality, but the black-coat color pig breed may have other genetic components for superior pork quality.
KEYWORD
MC1R, KNP, Polymorphism, Pork quality
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